Product Name |
Human sICAM-1/CD54(Soluble Intercellular Adhesion Molecule 1) ELISA Kit |
Catalogue No. |
EH0161 |
Synonyms |
sICAM-1 ELISA Kit, CD54 ELISA Kit, ICAM1 ELISA Kit, BB2 ELISA Kit, P3.58 ELISA Kit |
Detection Method |
Sandwich ELISA, Double Antibody |
Application |
sICAM-1/CD54 ELISA Kit allows for the in vitro quantitative determination of sICAM-1/CD54 concentrations in serum, plasma, tissue homogenates and other biological fluids. |
Size |
96T |
Range |
0.781-50ng/ml |
Sensitivity |
< 0.469ng/ml |
Species |
Human |
Standard Curve |
|
Storage |
2-8 ℃ for 6 months |
Recovery |
Matrices listed below were spiked with certain level of sICAM-1/CD54 and the recovery rates were calculated by comparing the measured value to the expected amount of sICAM-1/CD54 in samples.
Matrix |
Recovery range(%) |
Average(%) |
serum(n=5) |
89-103 |
94 |
EDTA plasma(n=5) |
90-103 |
96 |
heparin plasma(n=5) |
93-104 |
99 |
|
Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sICAM-1/CD54 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
serum(n=5) |
85-102% |
96-105% |
85-105% |
EDTA plasma(n=5) |
87-98% |
88-100% |
87-97% |
heparin plasma(n=5) |
80-90% |
80-95% |
80-99% |
|
CV(%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
Note |
For Research Use Only |
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