Description
Standard Curve
Storage: 2-8 ℃ for 6 months
Recovery: Matrices listed below were spiked with certain level of IL-4 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-4 in samples.
| Matrix | Recovery range(%) | Average(%) |
|---|---|---|
| serum(n=5) | 93-101 | 96 |
| EDTA plasma(n=5) | 89-101 | 95 |
| heparin plasma(n=5) | 92-105 | 98 |
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=5) | 85-103% | 88-95% | 92-99% |
| EDTA plasma(n=5) | 83-91% | 82-95% | 83-96% |
| heparin plasma(n=5) | 80-100% | 82-90% | 81-98% |
CV(%)Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only
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