This kit is used to detect the total antioxidant levels of antioxidants and antioxidant enzymes in the samples. It is mainly used in the study of biological, medical and pharmaceutical studies to detect the total antioxidant capacity of antioxidant solutions. In acid environment, Fe3+-TPTZ is reduced to blue Fe2+-TPTZ, this color reaction reflects the total antioxidant capacity.

The total antioxidant level is composed of various antioxidants and antioxidant enzymes in the test subject. In biological, medical, and pharmaceutical research, the total antioxidant capacity is often measured in various body fluids such as plasma, serum, saliva, and urine, as well as in lysates of cells or tissues, extracts of plants or Chinese herbal medicines, and solutions of various antioxidants.

Solution Preparation

• Extract solution: Pre cool on 2-8℃ refrigerator or ice.
• Standard: 10 mg of FeSO4·7H2O. Add 0.9 mL of distilled water and 20 μL of concentrated sulfuric acid (H2SO4) to forms 40 µmol/mL FeSO4 standard solution.
• Mixed solution: According to the sample size, mix Reagent I, Reagent II, and Reagent
  III=700μL:100μL: 100μL(900μL,5T). Prepare it for immediate use.

Technical Indicators

• Minimum detection limit: 0.000567243 μmol/mL
• Linear range: 0.00078125-0.1 μmol/mL