Description
Micro Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids. In the assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm.
Catalogue No. | 0301KTB |
Size | 96T |
Applicable samples | Serum, Plasma, Animal and Plant Tissues, Cells |
Kit components | • Assay Buffer
•Sample Diluent •Lysis Buffer (5×) •WST-8 |
Features & Benefits | • Determination of SOD enzyme activity in serum, plasma, plant or animal tissue、cell lysates and other biological fluids.
• Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors. |
Usage notes | • Briefly centrifuge small vials at low speed prior to opening.
• Prior to assay, bring all reagents to room temperature (25°C). The Xanthine reagent may appear to be turbid. Briefly vortext this tube before pipetting. • If not assayed immediately, samples can be stored at -80°C. |
Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information. |
Documents | Product manual with the material |
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