Description
Standard Curve
Storage: 2-8 ℃ for 6 months
Recovery: Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.
| Matrix | Recovery range(%) | Average(%) |
|---|---|---|
| serum(n=5) | 85-103 | 93 |
| EDTA plasma(n=5) | 86-104 | 95 |
| heparin plasma(n=5) | 88-104 | 94 |
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=5) | 85-104% | 88-103% | 87-104% |
| EDTA plasma(n=5) | 87-101% | 84-100% | 82-99% |
| heparin plasma(n=5) | 87-100% | 90-93% | 82-99% |
CV(%)Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only
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