| Detection Wavelength |
OD450 |
| Reaction Duration |
4 hours |
| Storage |
2-8°C(Sealed), Don’t cryopreserve. |
| Specificity |
Specifically binds with Ppargc1a , no obvious cross reaction with other analogues. |
| Standard Curve |
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
| STD.(ng/ml) |
OD-1 |
OD-2 |
Average |
| 0 |
0.124 |
0.128 |
0.126 |
| 0.156 |
0.172 |
0.176 |
0.174 |
| 0.312 |
0.202 |
0.208 |
0.205 |
| 0.625 |
0.288 |
0.296 |
0.292 |
| 1.25 |
0.431 |
0.443 |
0.437 |
| 2.5 |
0.675 |
0.695 |
0.685 |
| 5 |
1.234 |
1.27 |
1.252 |
| 10 |
2.127 |
2.189 |
2.158 |
|
| Recovery |
Add a certain amount of Ppargc1a into the sample. Calculate the recovery by comparing the measured value with the expected amount of Ppargc1a in the sample.
| Sample Type |
Recovery Range(%) |
Average(%) |
| serum(n=10) |
86-104 |
96 |
| EDTA plasma(n=10) |
85-97 |
91 |
| Heparin plasma(n=10) |
87-103 |
97 |
|
| Linearity |
Dilute the sample with a certain amount of Ppargc1a at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type |
1:2 |
1:4 |
1:8 |
| serum(n=10) |
86-99% |
86-101% |
87-104% |
| EDTA plasma(n=10) |
85-97% |
83-97% |
85-97% |
| Heparin plasma(n=10) |
82-99% |
80-96% |
86-99% |
|
| Precision(%) |
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item |
Intra-assay Precision |
Inter-assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean (ng/ml) |
0.32 |
1.24 |
5.09 |
0.31 |
1.2 |
5.2 |
| Standard deviation |
0.02 |
0.07 |
0.28 |
0.02 |
0.07 |
0.27 |
| CV(%) |
6.2 |
5.72 |
5.54 |
6.32 |
5.86 |
5.25 |
|
| Stability |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) |
37°C for 1 month |
2-8°C for 6 months |
| Average(%) |
80 |
95-100 |
|
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