Description
| Product name: | Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | 8OHdG; 7,8-Dihydro-8-Oxo-2′-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2′-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 26.29 pg/mL |
| Standard: | 6000 pg/mL |
| Range: | 93.75-6000 pg/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Hydroxydeoxyguanosine(8-OHdG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Hydroxydeoxyguanosine(8-OHdG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-Hydroxydeoxyguanosine(8-OHdG) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Standard Curve | 
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Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 78-92% | 85% |
| EDTA plasma(n=5) | 87-99% | 93% |
| Heparin plasma(n=5) | 80-95% | 97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 81-94% | 96-105% | 88-106% | 93-102% |
| EDTA plasma(n=5) | 95-104% | 97-106% | 85-98% | 81-92% |
| Heparin plasma(n=5) | 95-106% | 87-105% | 92-101% | 79-90% |
| Note | For Research Use Only |
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