Description
| Alternative Names | Human Tumor necrosis factor α,TNF-α ELISA KIT |
| Catalogue No. | 1761Hu |
| Size | 96T |
| Reactivity | Human |
| Assay range | 6pg/mL – 300pg/mL |
| Sensitivity | 2.2pg/ml |
| Sample Volume | 50ul |
| Wavelength | 450nm |
| Purpose | Our Human Tumor necrosis factor α,TNF-α ELISA KIT is to assay TNF-α levels in Human serum, plasma, culture media or any biological fluid. |
| Principle | This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNF-α Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for TNF-α is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-α and HRP conjugated TNF-α antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve. |
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