Description
| Product Name | HNF1A Antibody |
| Catalogue No. | DF6278 |
| Size | 100ul |
| Form | liquid |
| Purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). |
| Source: | Rabbit |
| Clonality | Polyclonal |
| Specificity: | Ferritin Heavy Chain Antibody detects endogenous levels of total Ferritin Heavy Chain. |
| Conjugate: | Unconjugated. |
| Storage | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. |
Description:
| Ferritin (FTH) is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain and ferritin light chain, each having unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, heavy chain ferritin also enhances thymidine biosynthesis (5). Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia. The level of serum ferritin is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7). |
IMAGES
| Western blot analysis of extracts from various samples, using Ferritin Heavy Chain Ab. Lane 1: Hepg2 cells(serum starvation treatment), blocked with antigenspecific peptides. Lane 2: Hepg2 cells(serum starvation treatment). Lane 3: Hela cells(heat-shock treatment). |
 |
| DF6278 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab. |
|
 |
Reviews
There are no reviews yet.