Description
Micro Catalase Activity Assay Kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.samples to the standard curve.
| Catalogue No. | 0301KTB |
| Size | 96T |
| Detection range | 2-75 µM |
| Sensitivity | 2 µM |
| Applicable samples | Serum, Plasma, Animal and Plant Tissues, Cells |
| Kit components | • Assay Buffer
• Sample Diluent • Formaldehyde standard (4.25 M) • Catalase (control) • Potassium Hydroxide • Hydrogen Peroxide • Chromogen • Potassium Periodate |
| Features & Benefits | • Determination of catalase activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining catalase activity directly by utilizing the peroxidatic function of catalase, which can not be interfered by other peroxidases. • A broad range linearity: 2-75 µM. • Measure catalase activity down to 2 U/ml |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C.
• Overheating can inactivate catalse. The enzyme should be kept cold during sample preparation and assaying. • In general, catalase is very unstable at high dilution. It is recommned to store samples concentrated and assay within 30 minutes afrer dilution. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information. |
| Documents | Product manual with the material |
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