Description
Features
Specifications
Species
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection method
Competitive ELISA, Coated with Antigen
Detection Wavelength
OD450
Reaction Duration
2 hours
Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Storage
2-8°C(Sealed), Don’t cryopreserve.
Kit Components
Item
Size(48T)
Size(96T)
E001
ELISA Microplate(Dismountable)
8×6
8×12
E002
Lyophilized Standard
1vial
| 2vial |
E003
Biotin-labeled Antibody(Concentrated, 100X)
30ul
60ul
E034
HRP-Streptavidin Conjugate(SABC, 100X)
60ul
| 120ul |
E024
| TMB Substrate |
5ml
| 10ml |
E039
| Sample Dilution Buffer |
10ml
| 20ml |
E040
| Antibody Dilution Buffer |
5ml
| 10ml |
E049
SABC Dilution Buffer
5ml
| 10ml |
E026
| Stop Solution |
5ml
| 10ml |
E038
| Wash Buffer(Concentrated, 25X) |
| 15ml |
| 30ml |
E006
Plate Sealer
3 pieces
5 pieces
E007
| Product Description |
| 1 copy |
1 copy
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item |
|---|
| Intra-assay Precision |
|---|
Inter-assay Precision
Sample
n
Mean(pg/mL)
Standard deviation
C V (%)
1
2
3
20
20
20
2.92
13.76
49.74
0.14
0.67
2.33
4.69
4.89
4.69
1
2
3
20
20
20
3.04
13.12
51.05
0.14
0.71
3.01
4.69
5.43
5.89
Add a certain amount of Glargine Neutralizing antibody into the sample. Calculate the recovery by comparing the measured value with the expected amount of Glargine Neutralizing antibody in the sample.
Sample Type
Range(%)
Average Recovery(%)
Serum (n=8)
91-105
97
EDTA plasma (n=8)
| 86-103 |
90
Heparin plasma(n=10)
89-103
97
Dilute the sample with a certain amount of Glargine Neutralizing antibody at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type |
|---|
| 1:2 |
|---|
| 1:4 |
|---|
| 1:8 |
|---|
serum(n=10)
90-104%
82-97%
81-99%
EDTA plasma(n=10)
91-105%
83-99%
80%97%
Heparin plasma(n=10)
89-103%
83-101%
87-97%
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