Description
Features
Specifications
Species
Human
Assay Type
Competitive Inhibition
Sensitivity
5.16 pmol/mL
Standard
1000 pmol/mL
Detection Range
15.63-1000 pmol/mL
Sample type
serum, plasma
Assay Length
2h
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
(pg/mL)
OD
Corrected
10.00
2.481
Human
2.430
1.601
1.550
2.50
0.908
0.857
1.25
0.473
0.422
0.63
0.264
0.213
0.31
0.158
0.107
0.16
0.107
0.056
0.00
0.051
0.000
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrices listed below were spiked with certain level of recombinant GA and the recovery rates were calculated by comparing the measured value to the expected amount of GA in samples.
Matrix
Range(%)
Average Recovery(%)
serum(n=5)
88-99%
93%
EDTA plasma(n=5)
80-97%
88%
Heparin plasma(n=5)
82-95%
91%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GA and their serial dilutions.
Matrix
1:2
1:4
1:8
1:16
serum(n=5)
86-97%
88-95%
79-93%
89-97%
EDTA plasma(n=5)
92-105%
83-92%
89-98%
85-94%
Heparin plasma(n=5)
85-94%
87-103%
97-105%
79-90%
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