Description
Species
Human
Assay Length
3h
Test Method
Double-antibody Sandwich
Sensitivity
The minimum detectable dose of this kit is typically less than 6.9pg/mL.
Detection Range
15.6-1,000pg/mL
Sample type
serum, plasma, tissue homogenates and other biological fluids
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Matrices listed below were spiked with certain level of recombinant Fibronectin Type III Domain Containing Protein 5 (FNDC5) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibronectin Type III Domain Containing Protein 5 (FNDC5) in samples.
Matrix
Recovery Range(%)
Average (%)
serum(n=5)
98-105
101
EDTA plasma(n=5)
81-94
84
Heparin plasma(n=5)
87-96
91
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibronectin Type III Domain Containing Protein 5 (FNDC5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
85-104%
79-99%
84-99%
83-97%
EDTA plasma(n=5)
89-98%
80-94%
92-99%
86-101%
Heparin plasma(n=5)
78-94%
80-90%
85-93%
98-105%
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