Description
Species
Human
Assay Length
3h
Test Method
Double-antibody Sandwich
Sensitivity
The minimum detectable dose of this kit is typically less than 0.237ng/mL.
Detection Range
0.625-40ng/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myostatin (MSTN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myostatin (MSTN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Matrices listed below were spiked with certain level of recombinant Myostatin (MSTN) and the recovery rates were calculated by comparing the measured value to the expected amount of Myostatin (MSTN) in samples.
Matrix
Recovery Range(%)
Average (%)
serum(n=5)
78-103
88
EDTA plasma(n=5)
89-96
93
Heparin plasma(n=5)
92-99
95
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myostatin (MSTN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
81-96%
91-101%
81-96%
97-105%
EDTA plasma(n=5)
84-101%
87-103%
91-98%
80-101%
Heparin plasma(n=5)
85-99%
91-105%
78-101%
92-105%
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