Description
Species
Human
Assay Length
3h
Test Method
Double-antibody Sandwich
Sensitivity
The minimum detectable dose of this kit is typically less than 34pg/mL.
Detection Range
78-5,000pg/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Matrices listed below were spiked with certain level of recombinant Intercellular Adhesion Molecule 1 (ICAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Intercellular Adhesion Molecule 1 (ICAM1) in samples.
Matrix
Recovery Range(%)
Average (%)
serum(n=5)
90-104
96%
EDTA plasma(n=5)
79-91
78%
Heparin plasma(n=5)
78-101
87%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intercellular Adhesion Molecule 1 (ICAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
94-105%
98-105%
94-102%
84-95%
EDTA plasma(n=5)
85-102%
89-96%
97-104%
91-101%
Heparin plasma(n=5)
83-101%
86-103%
94-102%
79-104%
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