Description

Specifications

Species

Human

Assay Length

3h

Test Method

Double-antibody Sandwich

Sensitivity

The minimum detectable dose of this kit is typically less than 34pg/mL.

Detection Range

78-5,000pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Rate of recovery

Matrices listed below were spiked with certain level of recombinant Intercellular Adhesion Molecule 1 (ICAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Intercellular Adhesion Molecule 1 (ICAM1) in samples.

Matrix

Recovery Range(%)

Average (%)

serum(n=5)

90-104

96%

EDTA plasma(n=5)

79-91

78%

Heparin plasma(n=5)

78-101

87%

Linear

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intercellular Adhesion Molecule 1 (ICAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

94-105%

98-105%

94-102%

84-95%

EDTA plasma(n=5)

85-102%

89-96%

97-104%

91-101%

Heparin plasma(n=5)

83-101%

86-103%

94-102%

79-104%