Description
Species
Human
Assay Length
2h
Test Method
Competitive Inhibition
Sensitivity
The minimum detectable dose of this kit is typically less than 2.71pg/mL.
Detection Range
6.17-500pg/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Matrices listed below were spiked with certain level of recombinant Endothelin 1 (EDN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Endothelin 1 (EDN1) in samples.
Matrix
Recovery Range(%)
Average (%)
serum(n=5)
88-98
92
EDTA plasma(n=5)
88-95
92
Heparin plasma(n=5)
78-105
92
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endothelin 1 (EDN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
97-104%
90-99%
98-105%
80-94%
EDTA plasma(n=5)
93-104%
96-103%
83-96%
95-103%
Heparin plasma(n=5)
79-90%
80-92%
89-102%
88-95%
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