Description
Species
Human
Assay Length
3h
Test Method
Double-antibody Sandwich
Sensitivity
The minimum detectable dose of this kit is typically less than 0.33ng/mL.
Detection Range
0.78-50ng/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Matrices listed below were spiked with certain level of recombinant Vascular Cell Adhesion Molecule 1 (VCAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Cell Adhesion Molecule 1 (VCAM1) in samples.
Matrix
Recovery Range(%)
Average (%)
serum(n=5)
85-99
95
EDTA plasma(n=5)
80-103
92
Heparin plasma(n=5)
97-104
101
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intercellular Adhesion Molecule 1 (ICAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
79-91%
95-103%
81-93%
79-90%
EDTA plasma(n=5)
98-105%
81-105%
99-105%
95-102%
Heparin plasma(n=5)
85-96%
78-105%
80-103%
93-103%
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