Description
Features
Specifications
Species
Human
Assay Length
3h
Test Method
Double-antibody Sandwich
Sensitivity
The minimum detectable dose of this kit is typically less than 5.5pg/mL.
Detection Range
15.6-1,000pg/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intra-assay Precision (Precision within an assay)：3 samples with low, middle and high level Stem Cell Factor (SCF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Matrices listed below were spiked with certain level of recombinant Stem Cell Factor (SCF) and the recovery rates were calculated by comparing the measured value to the expected amount of Stem Cell Factor (SCF) in samples.
Matrix
Range(%)
Average Recovery(%)
serum(n=5)
84-105
96%
EDTA plasma(n=5)
| 83-95 |
90%
Heparin plasma(n=5)
97-105
102%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stem Cell Factor (SCF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
80-104%
96-105%
94-102%
79-95%
EDTA plasma(n=5)
88-95%
78-101%
83-101%
98-105%
Heparin plasma(n=5)
78-104%
91-98%
84-93%
93-102%
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