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Human GA(Glycated Albumin) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GA protein.

SKU: BS-Eb-ELK8432 Categories: , Tag:

Description

Features
Specifications

Species

Human

Assay Type

Competitive Inhibition

Sensitivity

5.16 pmol/mL

Standard

1000 pmol/mL

Detection Range

15.63-1000 pmol/mL

Sample type

serum, plasma

Assay Length

2h

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL)
OD
Corrected

10.00

2.481

Human

2.430

1.601

1.550

2.50

0.908

0.857

1.25

0.473

0.422

0.63

0.264

0.213

0.31

0.158

0.107

0.16

0.107

0.056

0.00

0.051

0.000

Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Matrices listed below were spiked with certain level of recombinant GA and the recovery rates were calculated by comparing the measured value to the expected amount of GA in samples.

Matrix
Range(%)
Average Recovery(%)

serum(n=5)

88-99%

93%

EDTA plasma(n=5)

80-97%

88%

Heparin plasma(n=5)

82-95%

91%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GA and their serial dilutions. 

Matrix
1:2
1:4
1:8
1:16

serum(n=5)

86-97%

88-95%

79-93%

89-97%

EDTA plasma(n=5)

92-105%

83-92%

89-98%

85-94%

Heparin plasma(n=5)

85-94%

87-103%

97-105%

79-90%

Additional Information

pack

48 Test, 96 Test

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