Description
Features
Specifications
Species
Human
Uniprot ID
P01584
Detection method
Sandwich
Sensitivity
0.09 pg/mL
Standard
| 10pg/mL |
Detection Range
0.16-10pg/mL
Sample type
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time
3.5H
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
(pg/mL)
OD
Corrected
10.00
2.481
Human
2.430
1.601
1.550
2.50
0.908
0.857
1.25
0.473
0.422
0.63
0.264
0.213
0.31
0.158
0.107
0.16
0.107
0.056
0.00
0.051
0.000
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
(pg/mL)
(pg/mL)
Sample
n
Mean(pg/mL)
Standard deviation
C V (%)
1
2
3
20
20
20
1.16
4.77
1.75
0.64
4.59
8.76
6.98
6.29
5.64
1
2
3
20
20
20
8.69
6.85
4.44
0.67
3.27
2.12
4.19
5.32
5.56
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type
Range(%)
Average Recovery(%)
Serum (n=8)
81-95
88
EDTA plasma (n=8)
80-95
86
Cell culture media (n=8)
83-97
90
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
Average (%)
95-103
101
95-104
99
98-105
102
1:4
Range (%)
Average (%)
96-102
99
95-104
103
95-112
104
1:8
Range (%)
Average (%)
99-107
101
97-111
105
92-106
99
1:16
Range (%)
Average (%)
91-103
100
96-107
103
92-110
104
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