| Catalogue No. |
3806HE |
| Size |
96T |
| Synonyms |
SPA ELISA Kit, Pulmonary Surfactant Associated Protein A ELISA Kit, SFTPA1 ELISA Kit, COLEC4 ELISA Kit, PSAP ELISA Kit, SFTP1 ELISA Kit, SFTPA ELISA Kit, SFTPA1B ELISA Kit, PSP-A ELISA Kit, PSPA ELISA Kit, SP-A ELISA Kit, SP-A1 ELISA Kit, Collectin-4 ELISA Kit, 35 kDa pulmonary surfactant-associated protein ELISA Kit, Alveolar proteinosis protein ELISA Kit |
| Species |
Human |
| Range |
15.625-1000pg/ml |
| Sensitivity |
< 9.375pg/ml |
| Detection Method |
Sandwich ELISA, Double Antibody |
| Application |
SPA ELISA Kit allows for the in vitro quantitative determination of SPA concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Standard Curve |
 |
| Storage |
2-8 ℃ for 6 months |
| Recovery |
Matrices listed below were spiked with certain level of SPA and the recovery rates were calculated by comparing the measured value to the expected amount of SPA in samples.
| Matrix |
Recovery range(%) |
Average(%) |
| serum(n=5) |
89-102 |
97 |
| EDTA plasma(n=5) |
90-98 |
92 |
| heparin plasma(n=5) |
93-103 |
98 |
|
| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample |
1:2 |
1:4 |
1:8 |
| serum(n=5) |
85-103% |
85-101% |
91-103% |
| EDTA plasma(n=5) |
82-101% |
87-97% |
85-98% |
| heparin plasma(n=5) |
80-95% |
80-88% |
85-97% |
|
| CV(%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
| Note |
For Research Use Only |
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