Description

Product Name

Mouse MCSF(Colony Stimulating Factor 1, Macrophage) ELISA Kit

Catalogue No.

9611ELK

Synonyms

CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1

Assay Type

Sandwich

Sensitivity

0.59 pg/mL

Range

15.63-1000 pg/mL

Sample Type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length

3.5h

Research Area

Cytokine; Infection immunity;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 1, Macrophage(MCSF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Colony Stimulating Factor 1, Macrophage(MCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 1, Macrophage(MCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 1, Macrophage(MCSF) in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
1000.00	2.219	2.095
500.00	1.636	1.512
250.00	1.225	1.101
125.00	0.782	0.658
62.50	0.586	0.462
31.25	0.339	0.215
15.63	0.227	0.103
0.00	0.124	0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MCSF and the recovery rates were calculated by comparing the measured value to the expected amount of MCSF in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	80-95%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MCSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.