Description
Product Name |
3-NT(3-Nitrotyrosine) ELISA Kit |
Catalogue No. |
0652UE |
Detection Method |
Sandwich ELISA, Double Antibody |
Application |
3-NT ELISA Kit allows for the in vitro quantitative determination of 3-NT concentrations in serum, plasma, tissue homogenates and other biological fluids. |
Species |
Universal |
Standard Curve |
|
Storage |
2-8 ℃ for 6 months |
Recovery |
Matrices listed below were spiked with certain level of 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.
Matrix |
Recovery range(%) |
Average(%) |
serum(n=5) |
91-103 |
99 |
EDTA plasma(n=5) |
85-105 |
97 |
heparin plasma(n=5) |
89-101 |
95 |
|
Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
serum(n=5) |
85-105% |
93-105% |
93-105% |
EDTA plasma(n=5) |
88-101% |
82-100% |
93-101% |
heparin plasma(n=5) |
81-94% |
81-100% |
87-99% |
|
CV (%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
Note |
For Research Use Only |
Contact us for help
info@bio-string.com |
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